Bradford Reagent 5x

Bradford Reagent 5x is a known reagent used in Bradford assay. It is the mostly used colorimetric assay to determine the concentration of proteins. The procedure is based on the formation of a complex between the dye Brilliant Blue G and protein in a solution at acidic pH. The colorimetric reaction depends on the content of aromatic and basic amino acids. The protein-dye complex causes a shift in the maximum absorption of the dye from 465 to 595 nm. Absorbance increase is proportional to the amount of protein.
The protein concentration of an unknown sample can be determined using a calibration curve. To create the curve, it is recommended to use BSA (bovine serum albumin) as standard. Linear concentration range is 0.1-1.4 mg/mL of protein.

Research Use Only. Not for use in diagnostic procedures.