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Acrylamide/ bisacrylamide Solution 30%

42,00 99,00 

(37.5:1)

Overview

Polyacrylamide gel electrophoresis (PAGE) is probably the most common analytical technique used to separate and characterize proteins. Polyacrylamide gels are chemically crosslinked gels formed by the reaction of acrylamide with a bifunctional crosslinking agent such as N,N′-methylenebisacrylamide (Bis). The composition of the gel is given by %T, the total (w/v) concentration of acrylamide plus crosslinker, and %C, the (w/w) percentage of crosslinker included in %T.
The ‘pore size’ is determined by the ratio of acrylamide to bisacrylamide, and by the concentration of acrylamide. A high ratio of bisacrylamide to acrylamide and a high acrylamide concentration cause low electrophoretic mobility. Polymerization of acrylamide and bisacrylamide monomers is induced by ammonium persulfate (APS), which spontaneously decomposes to form free radicals. TEMED, a free radical stabilizer, is generally included to promote polymerization.
High Purity and ready-to-use Acrylamide/ Bisacrylamide solution (30%, 37.5:1) is prepared from electrophoresis grade acrylamide and bis-acrylamide in ultrapure water. The product is passed through a 0.2 µm filter.
This Ready-to-use Acrylamide/Bis-acrylamide solution contains 29.22% (w/v) acrylamide and 0.78% (w/v) bis-acrylamide for a monomer to crosslinker ratio of 37.5:1 (2.6% crosslinker).

 

Research Use Only. Not for use in diagnostic procedures.

* If TIARIS distributor is available in your country – please inquire directly.

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