L-Alanyl-L-Glutamine

L-Alanyl-L-Glutamine 100x is a concentrated sterile solution of stable L-glutamine, used as a supplement in cell culture medium. The dipeptide is metabolized within the cells to yield L-Glutamine plus the second amino acid. This results in a more consistent delivery of L-Glutamine to cells and avoids toxic build-up of ammonia in cell cultures. This feature can be especially important for ammonia-sensitive cell lines.
L-Alanyl-L-Glutamine prevents the intramolecular cyclization reaction associated with solutions of L-Glutamine, allowing the formulation of cell culture media containing L-Glutamine that may be stored at 4°C for extended periods. Solutions containing these derivatives can even be autoclaved without appreciable degradation of the product (30 minutes at 121°C results in <5% loss of the product).

L-glutamine is an essential amino acid that plays a key role in cellular metabolism, serving as a major source of nitrogen and carbon, which are essential for synthesizing proteins, nucleotides, and other molecules required for cell proliferation.
The concentration of L-glutamine needed varies depending on the cell type and medium formulation. Classical cell culture media like DMEM, GMEM, and IMDM typically contain L-glutamine concentrations ranging from 2 mM to 4 mM, which are adequate for most cell lines. However, certain cells, such as hybridoma cells, may require higher concentrations for optimal growth. In specialized media, such as serum-free or protein-free formulations, L-glutamine is often supplemented at levels around 2.5 mM to 6 mM.

Research Use Only. Not for use in diagnostic procedures.