Features
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cDNA synthesis and PCR in a single tube.
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100% inclusivity.
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Compatible with fast and standard PCR program
485,00 €
PhytoDETECT® ToMMV RT-PCR Kit enables an efficient cDNA synthesis and end-point PCR in a single tube. The kit includes an RT Master Mix, which contains a balanced mix of reverse transcriptase and RNase inhibitor, and a PCR Master Mix provided at 2x concentration for conducting PCR. Additionally, the kit contains a set of optimally designed primers for detecting the ToMMV virus via agarose gel electrophoresis.
The kit includes a positive control (PAC) to demonstrate that PCR amplification is being efficiently performed with the supplied components. To confirm the absence of contamination, a negative control reaction should be included each time the kit is used.
The Tomato Mottle Mosaic Virus (ToMMV) is one of the most recently characterized species of the Tobamovirus genus. Although first described in 2013, it has shown rapid global spread. The virus’s complete genome consists of a positive-sense, single-stranded RNA molecule. The virus infects tomatoes and peppers, causing symptoms such as leaf distortion, mosaic patterns, mottling, and necrosis. The virus is mechanically transmitted, through infected soil, and to a high degree through seeds.
Research Use Only. Not for use in diagnostic procedures.
* If TIARIS distributor is available in your country – please inquire directly.
Componentes |
TBK1072 |
PCR Master Mix (2x) | 1 mL |
ROX Reference | 1 vial |
RT Mix | 100 µL |
ToMMV Primers & Probe Mix (10x) | 200 µL |
ToMMV_PC (Positive Control) | 1 vial |
Water, nuclease free | 1 mL |
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