Q-PLUS™-Probe Real Time qPCR Master Mix (2x)

Q-PLUS™-Probe Real Time qPCR Master Mix, without ROX is an optimized Master Mix to be used in real time quantitative PCR. The detection involves the prevention of fluorescence of a fluorophore by the close proximity with a quencher and the 5’–3′ exonuclease activity of Taq polymerase to cleave the dual-labeled probe. The fluorescent signal intensity detected is proportional to the number of amplicons. The Master contains an antibody-based hotstart DNA polymerase, dNTPs, MgCl2, PCR enhancers and stabilization compound.

This kit also includes a separate vial of ROX that can be optionally added to the qPCR reaction Mix. The final concentration of ROX will vary depending on each real-time cycler manufacturer’s specification.

For Research Use Only. Not for use in diagnostic procedures.