Q-PLUS™ Universal Probe qPCR Master Mix (2X)

Overview

The Q-PLUS™ Universal Probe qPCR Master Mix (2X) is an optimized, ready-to-use kit designed for Real-time PCR (qPCR) with all standard qPCR machines. This Master Mix is provided as a 2× reaction mixture that contains all the necessary components required for real-time PCR, including dNTPs, stabilizers, and enhancers.
The Q-PLUS™ Probe qPCR Master Mix (2X) contains a Hot-Start Taq DNA Polymerase that has been chemically modified. This polymerase is inactive at room temperature, which helps to prevent the extension of non-specifically annealed primers or primer dimers, leading to a higher specificity of DNA amplification.
In addition, this kit includes a tube containing Internal Control (IC) and a tube containing IC primers/Probe (YY-TAMRA). The IC included in the Master Mix serves to verify that the PCR procedure was done correctly.
This kit also includes a separate vial of ROX that can be optionally added to the qPCR reaction Mix. The final concentration of ROX will vary depending on each real-time cycler manufacturer’s specification.

For Research Use Only. Not for use in diagnostic procedures.