ROS Detection Assay Kit

ROS Detection Assay Kit is a widely used kit designed to detect reactive oxygen species (ROS), highly reactive molecules containing oxygen such as free radicals like superoxide anions (O₂•⁻), hydroxyl radical (HO•) and non-radical compounds like hydrogen peroxide (H₂O₂). ROS are expressed in various cellular compartments including peroxisomes, mitochondria and endoplasmic reticulum. These molecules play essential roles in cell signaling and homeostasis but can cause oxidative stress when present in excess, leading to cellular damage.

ROS Detection Assay Kit is based on the use of the fluorogenic substrate H₂DCFDA. H₂DCFDA is a cell-permeable, non-fluorescent compound that, once inside the cell, is deacetylated by intracellular esterase to H₂DCF. In the presence of ROS, H₂DCF is then oxidized to the highly fluorescent compound DCF (dichlorofluorescein). The intensity of DCF fluorescence can be measured providing a reliable indicator of intracellular ROS levels.

Research Use Only. Not for use in diagnostic procedures.