Features
- Efficient long targets amplifications (5-20 kb).
- PCR fragments suitable to be cloning in TA-vectors or blunt vectors.
- Increased yield and fidelity of PCR products.
- Error Rate 5.6 x10−6 errors/bp per cycle.
5,00 € – 225,00 €
Overview
TOO™ LONG DNA Polymerase Kit is a convenient kit that includes TOO™ LONG DNA polymerase and highly pure High-Q™ dNTPs to enable the amplification of targets since 5 kb to 20 kb.
TOO™ LONG DNA polymerase is a blended enzyme preparation which combines a polymerase with 3′-5′ exonuclease activity with a polymerase lacking 3′-5′ exonuclease activity. PCR amplification generates a mixture of A-overhang-ended (predominantly) and blunt-ended PCR products.
Research Use Only. Not for use in diagnostic procedures.
* If TIARIS distributor is available in your country – please inquire directly.
Components | TBK0046 | TBK0047 |
TOO™ LONG DNA polymerase (5 U/ µL) | 100 μL | 2 x 100 μL |
TOO™ LONG PCR Buffer (10x) | 1.5 mL | 2 x 1.5 mL |
MgCl2 25 mM | 1.5 mL | 2 x 1.5 mL |
High-Q™ dNTPs 10 mM TOTAL | 1 mL | 2 x 1 mL |
DMSO (100%) | 50 µL | 50 µL |
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