Tris-Tricine-SDS Buffer 10x, pH 8.3

Tris-Tricine-SDS Buffer 10x, pH 8.3

Overview

Tris-Tricine-SDS Buffer 10x, pH 8.3 is the perfect buffer in the separation of peptides, low molecular weight proteins and hydrophobic proteins. Replacement of traditional glycine (pK 9.6) with Tricine (pK 8.15) allows a better resolution of low molecular weight proteins when compared to the Laemmli method. Besides, Tricine gel are particularly suitable for isolating hydrophobic proteins from 2D gels, facilitates its transfer during Western blotting and it is effective in the isolation of protein complexes from biological membranes.

For Research Use Only. Not for use in diagnostic procedures.

Cat. No.Conc.SizePrice
TBB039510 X500 mL59€
TBB039610 X1 L99€

Prices excl. VAT plus shipping costs.

How to order?

  • Sterile colorless liquid.
  • pH=8.3 ± 0.05.
  • Composition (10x): 1M Tris, 1M Tricine, 1% SDS, pH 8.3.
  • Protease not detected.
  • Buffering capacity in the range of pH 7.4-8.8.
  • Western Blot Transfer Buffer for hydrophobic proteins
  • Running Buffer for 2D Gel electrophoresis.
  • Mass spectrometric buffer.
  • Peptide separation.
  • Shipped in: Shipped at ambient temperature.
  • Storage: Store at room temperature (15-25ºC) 

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