Features
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Concentration: 1M
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pH: 6.5–7.5
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Osmolality: >1000 mOsm/kg
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Endotoxin: <0.05 EU/ml (at 25mM concentration)
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Sterility: sterile
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Preparation of working solution: Add directly to cell culture media to a final concentration of 25-40 mM

63,00 €
(Cell Biology Grade)
HEPES Buffer Solution (1M) is widely used as a buffering agent in cell culture media. The zwitterion possesses a pKa(1) of 3 and a pKa(2) of 7.5 with useful pH ranges of 2.5-3.5 and 6.7-8.6. In comparison with bicarbonate buffer systems, HEPES is better in maintaining physiological pH despite changes in CO2 concentrations resulting from cellular activity. HEPES is suitable for many cell culture systems as it is membrane impermeable and has limited effect on biochemical reactions.
Research Use Only. Not for use in diagnostic procedures.
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