Features
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High Efficiency in multiplex reactions
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High Efficiency in GC/AT-rich templates
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Early Ct values – Rapid extension rate
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Extreme sensitivity – increased limit of detection
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Compatible with fast and standard PCR program
10,00 € – 560,00 €Price range: 10,00 € through 560,00 €
Q-PLUS™ One-Step Probe RT-qPCR Master Mix (2x) allows first-strand cDNA synthesis and subsequent qPCR in a single-tube reaction procedure, reducing the risk of contamination and significantly minimizing hands-on time. The kit includes our Q-PLUS™ Probe qPCR Master Mix, presented as a 2× reaction mixture. This mix incorporates all essential components for real-time PCR, including dNTPs, stabilizers, and enhancers, designed for the efficient amplification and detection of DNA in qPCR based on a wide range of probe-based technologies, including Taqman®, Molecular Beacons® and Scorpion probes®. In addition, a separate RT mix that comprises a balanced mixture of both Reverse transcriptase and Ribonuclease Inhibitor is also provided.
This kit also includes a separate vial of ROX that can be optionally added to the qPCR reaction Mix. The final concentration of ROX will vary depending on each real-time cycler manufacturer’s specification.
Research Use Only. Not for use in diagnostic procedures.
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Components |
TBK0010 |
TBK0011 |
Q-PLUS™- Probe qPCR Master Mix, (2x) |
1 mL |
5 x 1 mL |
ROX reference dye |
1 vial |
1 vial |
RT Mix |
100 µL |
5 x 100 µL |
RNase-free Water |
1 mL |
5 x 1 mL |