TIARIS™ HRM FAST PCR Mastermix (2X)
Price range: 87,00 € through 400,00 €
The TIARIS™ SNP PCR Mastermix (2X) is based on a highly specific engineered Taq DNA polymerase variant and an optimized buffer system, both of which enable reliable and accurate allelic discrimination.
The modified Taq polymerase included in the mix is an aptamer-inhibited hot-start enzyme, specifically designed for applications requiring high single-nucleotide discrimination — for instance, in allele-specific amplifications (ASA) by PCR, primer extension assays, methylation-specific PCR (MSP), HLA genotyping, or the analysis of single CpG methylation sites. The enzyme incorporates a special N-terminal deletion and proprietary amino acid substitutions within its active site. These modifications increase its sensitivity to mismatches at the 3′ end of primers, significantly enhancing specificity.
As a result, non-specific amplicons are minimized when primers are not perfectly matched to the target sequence. Additionally, Taq DNA Polymerase shows 5’-3’, especially applicable for usage together with hydrolysis probes.
Research Use Only. Not for use in diagnostic procedures.