Tris-Tricine-SDS Buffer, pH 8.3

Overview

Tris-Tricine-SDS Buffer, pH 8.3 is the perfect buffer in the separation of peptides, low molecular weight proteins and hydrophobic proteins. Replacement of traditional glycine (pK 9.6) with Tricine (pK 8.15) allows a better resolution of low molecular weight proteins when compared to the Laemmli method. Besides, Tricine gel are particularly suitable for isolating hydrophobic proteins from 2D gels, facilitates its transfer during Western blotting and it is effective in the isolation of protein complexes from biological membranes.