LDH Cytotoxicity Assay Kit

LDH Cytotoxicity Assay Kit is a robust kit to measure cell death or cytotoxicity in cell culture experiments through lactate dehydrogenase (LDH) activity. LDH is a cytosolic enzyme that is rapidly released into the cell medium after damage of cell membrane. The amount of LDH released is proportional to the number of cells undergoing necrosis, apoptosis, or other forms of cell death, as well as from damaged or stressed cells.

LDH activity determination is based on the reduction of the tetrazolium salt, iodonitrotetrazolium chloride (INT). INT is reduced by LDH in the supernatant to form a colored formazan product that can be quantified spectrophotometrically at 490 nm.

Research Use Only. Not for use in diagnostic procedures.