Trypsin-EDTA in DPBS

Trypsin-EDTA is a well-known solution used as standard cell detachment solution to detach cells from standard tissue culture plastic ware and adhesion coated plastic ware. It contains pancreatic porcine trypsin at 0.05% (1x) or 0.5% (10x) in DPBS pH=7.3 ± 0.3. Trypsin is a serine protease that hydrolyzes proteins at the carboxyl side of the Lysine or Arginine. EDTA presence enhances trypsin action, and reduces the required trypsin concentration for effective hydrolysis. The solution does not include calcium and magnesium.

Research Use Only. Not for use in diagnostic procedures.