Senescence Detection Kit

Senescence Detection Kit enables reliable identification of senescent cells in vitro by detecting the enzymatic activity of lysosomal senescence-associated β-galactosidase (SA-β-gal), a widely accepted specific biomarker of cellular senescence. This assay is based on the hydrolysis of substrate (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside), a chromogenic substrate, which yields an easily visible blue precipitate under light microscopy.
β-Galactosidase activity is significantly increased in senescent cells, allowing their clear identification even in heterogeneous cultures. The enzyme hydrolyzes the substrate into a colored product that accumulates within the cells, enabling visual detection of them.

Research Use Only. Not for use in diagnostic procedures.