Recombinant DNase-I Kit

(RNase free)

Overview

Recombinant DNase-I is an endonuclease from bovine pancreas produced in Pichia pastoris. It is a common reagent used to remove DNA in RNA and protein extraction protocols. The enzyme splits phosphodiester linkages within DNA releasing a mixture of oligo- and mononucleotides. DNase-I acts on single stranded DNA (ssDNA), double stranded DNA (dsDNA) and chromatin. DNase-I generates double-stranded breaks in Mn²⁺ presence, while in presence of Mg²⁺ the enzyme produces single-stranded nicks in dsDNA.

The Recombinant DNase-I can be used for both DNase digestion in solution as well as “in-column” digestion in combination with Tiaris Biosciences RNA Purification Kits.

For Research Use Only. Not for use in diagnostic procedures.