Products

DMEM/F-12 with L-Glutamine

35,00 €

(with Phenol Red and Sodium Pyruvate and without HEPES)

Overview

DMEM/F-12 is a 1:1 mixture of Dulbecco’s Modified Eagle Medium (DMEM) and Ham’s F-12 Nutrient Mixture, designed to combine the advantages of both formulations into a single, highly versatile basal medium.
The medium is supplemented with L-Glutamine, an essential amino acid required for optimal cell growth and metabolism.
DMEM/F-12 is widely used for the cultivation of a broad range of mammalian cells, including epithelial, endothelial, fibroblast, and neuronal cell types.

Research Use Only. Not for use in diagnostic procedures.

Volume

For bulk orders, OEM solutions, or customized formats, please contact our technical team.

Features
Features
- Filter sterilized DMEM/F-12 supplemented with L-Glutamine.
- Clear red/orange solution.
- This formulation is with Phenol Red and Sodium Pyruvate and without HEPES.
- pH: 7.0-7.6.
- Osmolality: 280-320 mOsm/kg.
- Endotoxin: <1.0 EU/ml
Applications
Applications
- Cell culture applications.
Documentation
Documentation
- Data Sheet
- MSDS
- Certificate of Analysis

Composition

Shipping and storage
Shipping and storage
- Store at +4°C, protected from light, for up to 12 months. Once the product has been opened, store at +4%C and use within 1 or 2 months.
- Shipment is typically carried out at room temperature as this product can be kept at
  room temperature for up to 2 weeks without any problem.

Amino Acids (mg/L)		Vitamins (mg/L)
Component	Concentration	Component	Concentration
Glycine	18.75	D-Biotin	0.0035
L-Alanine	4.45	Choline chloride	8.98
L-Arginine HCl	147.50	D-Calcium pantothenate	2.24
L-Asparagine H₂O	7.50	Folic Acid	2.66
L-Aspartic Acid	6.65	Myo-Inositol	12.60
L-Cysteine HCl H₂O	17.56	Nicotinamide	2.02
L-Cystine 2HCl	31.29	Pyridoxal HCl	2.00
L-Glutamic Acid	7.35	Pyridoxine HCl	0.031
L-Glutamine	365.00	Riboflavin	0.219
L-Histidine HCl H₂O	31.48	Thiamine HCl	2.17
L-Isoleucine	54.47	Vitamin B12	0.68
L-Leucine	59.05	Salts (mg/L)
L-Lysine HCl	91.25	Component	Concentration
L-Methionine	17.24	CaCl₂·2H₂O	154.50
L-Phenylalanine	35.48	CuSO₄·5H₂O	0.0013
L-Proline	17.25	Fe(NO₃)₃·9H₂O	0.05
L-Serine	26.25	FeSO₄·7H₂O	0.417
L-Threonine	53.45	KCl	311.80
L-Tryptophan	9.02	MgCl₂·6H₂O	61.20
L-Tyrosine 2Na 2H₂O	55.79	MgSO₄·7H₂O	100.00
L-Valine	52.85	NaCl	6996.00
Others (mg/L)		NaHCO₃	1200.00
Component	Concentration	Na₂HPO₄	71.02
D-Glucose	3151.00	NaH₂PO₄·2H₂O	70.87
Phenol Red (Na)	8.63	ZnSO₄·7H₂O	0.432
Hypoxanthine	2.40
Linoleic Acid	0.042
Lipoic Acid	0.105
Putrescine 2 HCl	0.081
Sodium Pyruvate	55.00
Thymidine	0.365

Products

DMEM/F-12 with L-Glutamine

(with Phenol Red and Sodium Pyruvate and without HEPES)

Overview

Features

Filter sterilized DMEM/F-12 supplemented with L-Glutamine.

Clear red/orange solution.

This formulation is with Phenol Red and Sodium Pyruvate and without HEPES.

pH: 7.0-7.6.

Osmolality: 280-320 mOsm/kg.

Endotoxin: <1.0 EU/ml

Applications

Cell culture applications.

Documentation

Data Sheet

MSDS

Certificate of Analysis

Composition

Shipping and storage

Related Products

Dulbecco Phosphate Buffer Saline (DPBS), 1X (w/o Ca, Mg and Phenol Red)

Dulbecco Phosphate Buffer Saline (DPBS)

Resazurin Viability Assay Kit

Trypsin-EDTA in DPBS

Products

Services

Resources and Support