Features
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Filter sterilized DMEM/F-12 supplemented with L-Glutamine.
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Clear red/orange solution.
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This formulation is with Phenol Red and Sodium Pyruvate and without HEPES.
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pH: 7.0-7.6.
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Osmolality: 280-320 mOsm/kg.
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Endotoxin: <1.0 EU/ml
35,00 €
DMEM/F-12 is a 1:1 mixture of Dulbecco’s Modified Eagle Medium (DMEM) and Ham’s F-12 Nutrient Mixture, designed to combine the advantages of both formulations into a single, highly versatile basal medium.
The medium is supplemented with L-Glutamine, an essential amino acid required for optimal cell growth and metabolism.
DMEM/F-12 is widely used for the cultivation of a broad range of mammalian cells, including epithelial, endothelial, fibroblast, and neuronal cell types.
Research Use Only. Not for use in diagnostic procedures.
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| Amino Acids (mg/L) | Vitamins (mg/L) | |||
| Component | Concentration | Component | Concentration | |
| Glycine | 18.75 | D-Biotin | 0.0035 | |
| L-Alanine | 4.45 | Choline chloride | 8.98 | |
| L-Arginine HCl | 147.50 | D-Calcium pantothenate | 2.24 | |
| L-Asparagine H₂O | 7.50 | Folic Acid | 2.66 | |
| L-Aspartic Acid | 6.65 | Myo-Inositol | 12.60 | |
| L-Cysteine HCl H₂O | 17.56 | Nicotinamide | 2.02 | |
| L-Cystine 2HCl | 31.29 | Pyridoxal HCl | 2.00 | |
| L-Glutamic Acid | 7.35 | Pyridoxine HCl | 0.031 | |
| L-Glutamine | 365.00 | Riboflavin | 0.219 | |
| L-Histidine HCl H₂O | 31.48 | Thiamine HCl | 2.17 | |
| L-Isoleucine | 54.47 | Vitamin B12 | 0.68 | |
| L-Leucine | 59.05 | Salts (mg/L) | ||
| L-Lysine HCl | 91.25 | Component | Concentration | |
| L-Methionine | 17.24 | CaCl₂·2H₂O | 154.50 | |
| L-Phenylalanine | 35.48 | CuSO₄·5H₂O | 0.0013 | |
| L-Proline | 17.25 | Fe(NO₃)₃·9H₂O | 0.05 | |
| L-Serine | 26.25 | FeSO₄·7H₂O | 0.417 | |
| L-Threonine | 53.45 | KCl | 311.80 | |
| L-Tryptophan | 9.02 | MgCl₂·6H₂O | 61.20 | |
| L-Tyrosine 2Na 2H₂O | 55.79 | MgSO₄·7H₂O | 100.00 | |
| L-Valine | 52.85 | NaCl | 6996.00 | |
| Others (mg/L) | NaHCO₃ | 1200.00 | ||
| Component | Concentration | Na₂HPO₄ | 71.02 | |
| D-Glucose | 3151.00 | NaH₂PO₄·2H₂O | 70.87 | |
| Phenol Red (Na) | 8.63 | ZnSO₄·7H₂O | 0.432 | |
| Hypoxanthine | 2.40 | |||
| Linoleic Acid | 0.042 | |||
| Lipoic Acid | 0.105 | |||
| Putrescine 2 HCl | 0.081 | |||
| Sodium Pyruvate | 55.00 | |||
| Thymidine | 0.365 | |||