ONPG β-Galactosidase Assay Kit

ONPG β-Galactosidase Assay Kit is an extraordinary tool commonly used in molecular biology and microbiology research to measure the activity of beta-galactosidase enzyme. This assay can be used to detect the presence of certain bacterial strains and to monitor expression of lacZ as β galactosidase reporter gene.

ONPG β-Galactosidase Assay Kit is a quantitative method based on the cleavage of the synthetic chromogenic lactose analogous substrate ortho-nitrophenyl-beta-galactopyranoside (ONPG), releasing a bright yellow product called ortho-nitrophenol (ONP). The ONP production per unit time is directly proportional to the activity of β-galactosidase in the sample.

Features

• Accurate, simple and reproducible assay, around 3,5-6% inter- and 3.3-5.5% intra- assay coefficient of variation.
• Narrow dynamic range, linearity from 0.2 to 2 nmol/min/mg protein.
• Colorimetric readout at 420 nm.
• Highly Sensitive, the assay can detect beta-galactosidase activity in the range of 0.1-10 nmol/min/mg protein.
• Proteolytic resistance of β-galactosidase, lysates could be assayed directly or store at -80 ºC for at least 2 months.

Applications

• Normalization of transfection efficiency in mammalian cells.
• Study of protein trafficking, strength of promoters and enhancer with β-galactosidase as reporter.
• Protein-protein interaction studies.

Research Use Only. Not for use in diagnostic procedures.