Acrylamide/ bisacrylamide Solution 30%

Polyacrylamide gel electrophoresis (PAGE) is probably the most common analytical technique used to separate and characterize proteins. Polyacrylamide gels are chemically crosslinked gels formed by the reaction of acrylamide with a bifunctional crosslinking agent such as N,N′-methylenebisacrylamide (Bis). The composition of the gel is given by %T, the total (w/v) concentration of acrylamide plus crosslinker, and %C, the (w/w) percentage of crosslinker included in %T.
The ‘pore size’ is determined by the ratio of acrylamide to bisacrylamide, and by the concentration of acrylamide. A high ratio of bisacrylamide to acrylamide and a high acrylamide concentration cause low electrophoretic mobility. Polymerization of acrylamide and bisacrylamide monomers is induced by ammonium persulfate (APS), which spontaneously decomposes to form free radicals. TEMED, a free radical stabilizer, is generally included to promote polymerization.
High Purity and ready-to-use Acrylamide/ Bisacrylamide Solution 30%  (37.5:1) is prepared from electrophoresis grade acrylamide and bis-acrylamide in ultrapure water. The product is passed through a 0.2 µm filter.
This Ready-to-use Acrylamide/Bis-acrylamide solution contains 29.22% (w/v) acrylamide and 0.78% (w/v) bis-acrylamide for a monomer to crosslinker ratio of 37.5:1 (2.6% crosslinker).

Research Use Only. Not for use in diagnostic procedures.