High-Q™ Spin-Column Stool DNA Purification Kit

High-Q™ Spin-Column Stool DNA Purification Kit provides a convenient method to isolate total DNA from fresh or frozen stool samples. The kit can also be used to isolate DNA from stool samples preserved using our Stool Collection and Preservation Kit.
The stool sample is homogenized and disrupted under denaturing conditions using a specially formulated lysis buffer. Humic acid, proteins, polysaccharides, and other contaminants are subsequently precipitated and removed using a proprietary inhibitor removal buffer. Genomic DNA in the sample is then bound by the Spin Column followed two rapid wash steps to remove trace contaminants, and pure DNA is eluted with Elution Buffer.
The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.