Lysozyme Detection Kit

(Turbidimetric method)

Overview

The Lysozyme Detection Kit provides ready-to-use reagents for detecting the presence of lysozyme activity. This simple assay detects lysozyme activity using Micrococcus luteus cells as the substrate.

Lysozyme, also known as muramidase or N-acetylmuramic hydrolase, is a small, monomeric enzyme that hydrolyzes the β-1,4-linkages between N-acetyl-d-glucosamine and N-acetylmuramic acid residues in the peptidoglycan of bacterial cell walls. The cell lysis, or bursting, that usually follows, is the basis for its bactericidal activity in vivo.
The assay method typically employed to measure lysozyme activity is based on the lysis of Micrococcus luteus cells following the hydrolysis of the substrate, the protective cell wall of this organism, by lysozyme.
The turbidimetric methods are among the simplest and quickest to perform and rely on the spectrophotometric measurement of the suspensions of M. luteus. During the incubation of the lysozyme sample and substrate (M. luteus), the reaction is followed by monitoring the decrease in absorbance at 450 nm.