TOO™ LONG DNA Polymerase Kit

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PCR Reagents & Kits

TOO™ LONG DNA Polymerase Kit

Overview

TOO™ LONG DNA Polymerase Kit is a convenient kit that includes TOO™ LONG DNA polymerase and highly pure High-Q™ dNTPs to enable the amplification of targets since 5 kb to 20 kb.
TOO™ LONG DNA polymerase is a blended enzyme preparation which combines a polymerase with 3′-5′ exonuclease activity with a polymerase lacking 3′-5′ exonuclease activity. PCR amplification generates a mixture of A-overhang-ended (predominantly) and blunt-ended PCR products.

Features

  • Efficient long targets amplifications (5-20 kb).
  • PCR fragments suitable to be cloning in TA-vectors or blunt vectors.
  • Increased yield and fidelity of PCR products.
  • Error Rate 5.6 x10−6 errors/bp per cycle.

For Research Use Only. Not for use in diagnostic procedures.

Cat. No.FormatConc.Price
TBK004520 U5 u/µL10€ (Test sample)*
TBK0046500 U 5 u/µL135€
TBK00471000 U 5 u/µL225€

*Test sample available! The test sample price will be refunded on the first official order of the product.

How to order?

ComponentsTBK0046TBK0047
TOO™ LONG DNA polymerase (5 U/ µL)100 μL2 x 100 μL
TOO™ LONG PCR Buffer (10x)1.5 mL2 x 1.5 mL
MgCl2 25 mM1.5 mL2 x 1.5 mL
High-Q™ dNTPs 10 mM TOTAL1 mL2 x 1 mL
DMSO (100%)50 µL50 µL
  • Suitable as a direct replacement for ordinary STOUT™ Recombinant Taq DNA Polymerase in most applications.
  • Generation of PCR fragments for TA or blunt cloning.
  • Sequencing.
  • DNA Labeling.
  • Amplifications of long target sequences.
  • Shipped in: Shipped in blue ice. 
  • Storage: Store at -20 °C.

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