Q-PLUS™ One-Step Probe RT-qPCR Master Mix (2x)

Q-PLUS™ One-Step Probe RT-qPCR Master Mix (2x) allows first-strand cDNA synthesis and subsequent qPCR in a single-tube reaction procedure, reducing the risk of contamination and significantly minimizing hands-on time. The kit includes our Q-PLUS™ Probe qPCR Master Mix, presented as a 2× reaction mixture. This mix incorporates all essential components for real-time PCR, including dNTPs, stabilizers, and enhancers, designed for the efficient amplification and detection of DNA in qPCR based on a wide range of probe-based technologies, including Taqman®, Molecular Beacons® and Scorpion probes®. In addition, a separate RT mix that comprises a balanced mixture of both Reverse transcriptase and Ribonuclease Inhibitor is also provided.

This kit also includes a separate vial of ROX that can be optionally added to the qPCR reaction Mix. The final concentration of ROX will vary depending on each real-time cycler manufacturer’s specification.