TIARIS™ HRM FAST PCR Mastermix (2X)

High Resolution Melt (HRM) analysis is a post-PCR technique based on the precise monitoring of double-stranded DNA dissociation through gradual temperature increases. It uses intercalating fluorescent dyes that emit signal only when bound to double-strand DNA, allowing the detection of subtle sequence differences through changes in melting profiles. Due to its high sensitivity, HRM has become a powerful tool in several applications, such as SNP genotyping, detection of somatic mutations, and DNA methylation analysis.

The TIARIS™ HRM FAST PCR Mastermix (2x) has been specifically optimized for high-resolution melting applications, offering excellent performance in terms of amplification efficiency, signal consistency, and mutation discrimination. The mix combines a highly efficient enzyme with an optimized buffer system, enabling robust amplification of both GC-rich and AT-rich templates under standard and fast qPCR cycling conditions. The intercalating dye used in this Mastermix does not inhibit PCR, allowing for exceptional sensitivity and specificity, while also preventing the formation of primer-dimers and non-specific products.