pSHARP™-TA PLUS Vector Cloning Kit

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Reagents

pSHARP™-TA PLUS Vector Cloning Kit

pSHARP™-TA PLUS Vector Cloning Kit is an excellent tool for cloning 3′-A ended fragments generated with Taq DNA polymerase or any other polymerase lacking of 3′ to 5′ proofreading activity. This fact is conveniently used to clone these PCR fragments into a complementary 3′-T overhang vector.
Lineal pSHARP™-TA PLUS Vector is included in the kit. It is prepared by an improved procedure that includes a blunt enzyme digestion and subsequent addition of 3′ thymidine by a transferase terminal activity.

Features

  • Ready to use linearized cloning vector
  • Improved vector, with minimal background
  • High cloning efficiency obtaining large number of white positive colonies
  • Recombinant positive clones are distinguished from closed vector by white/ blue screening
  • Easy and fast protocol
  • Cloning without restriction enzymes

For Research Use Only. Not for use in diagnostic procedures.

Cat. No.FormatPrice
TBK080610 reactions85€
TBK080720 reactions165€

Prices excl. VAT plus shipping costs.

How to order?

ReagentsTBK0806TBK0807
pSHARP™-TA PLUS vector (50 ng/ μL)10 µL20 µL
T4 DNA Ligase (5 U Weiss/ μL)10 µL20 µL
5x T4 DNA Ligase Buffer100 µL200 µL
Insert Control (30 ng/ μL)5 µL5 µL
  • Cloning of PCR fragments amplified with non-proofreading polymerases.
  • In vitro transcription from T7 or SP6 promoters.
  • Shipped in: Shipped in ice blue.
  • Storage: Store at -20°C.
     

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