pSHARP™-TA Vector Cloning Kit

Overview

pSHARP™-TA Vector Cloning Kit is an excellent tool for cloning 3′-A ended fragments generated with Taq DNA polymerase or any other polymerase lacking of 3′ to 5′ proofreading activity. This fact is conveniently used to clone these PCR fragments into a complementary 3′-T overhang vector.
Lineal pSHARP™-TA Vector is included in the kit. It is prepared by an improved procedure that includes a blunt enzyme digestion and subsequent addition of 3′ thymidine by a transferase terminal activity.

For Research Use Only. Not for use in diagnostic procedures.