TIARIS™ High-Fidelity DNA Polymerase kit

TIARIS™ High-Fidelity DNA polymerase is a robust enzyme with 3´→ 5´ exonuclease (proofreading) activity and enhanced DNA binding, resulting in improved processivity, yield, and extremely low error-rate. TIARIS™ High-Fidelity DNA polymerase has an error-rate of approximately 1 error per 4.4×10⁷ nucleotides incorporated, which is 50x lower than Taq DNA polymerase. The enzyme is ideal for applications such as high-fidelity PCR, site-directed mutagenesis, crude sample PCR, blunt-end cloning, among others, where robustness and proofreading are important. The supplied reaction buffer (5x) includes not only 15 mM MgCl₂, but also 5mM dNTPs, enhancers, and stabilizers, optimized to increased PCR success rates.