PCR Reagents & Kits
TIARIS™ Pfu DNA Polymerase Kit
Overview
TIARIS™ Pfu DNA Polymerase Kit is an ideal kit when high fidelity is an important requirement in your PCR. It includes the proofreading enzyme Tiaris™ Pfu DNA polymerase and the highly purity High-Q™ dNTPs. Tiaris™ Pfu DNA polymerase (90 kDa) is a high fidelity polymerase from Pyrococcus furiosus produced in Escherichia coli. The enzyme has a strong 5′ to 3′ polymerase activity and 3′ to 5′ exonuclease (proofreading) activity which corrects nucleotide incorporation errors, thereby increasing fidelity and accuracy of DNA polymerization.
Features
- High accuracy and fidelity PCR with an error rate of 1.3×10−6 errors/bp per cycle.
- Elongation velocity is 0.2~0.4 kb/min (70~75°C).
- Suitable for blunt cloning purposes, the enzyme produces blunt-end PCR fragments.
- Highly thermostable enzyme, which retains 94-99% of its activity after 1 hour at 95°C.
- PCR amplification > 2 kb require optimization.
For Research Use Only. Not for use in diagnostic procedures.
| Cat. No. | Format | Concentracion | Price |
| TBK0085 | 12.5 U | 2.5 u/µL | 10€ (Test sample)* |
| TBK0086 | 125 U | 2.5 u/µL | 65€ |
| TBK0087 | 500 U | 2.5 u/µL | 235€ |
*Test sample available! The test sample price will be refunded on the first official order of the product.
Prices excl. VAT plus shipping costs.
How to order?| Components | TBK0086 | TBK0087 |
| Tiaris™ Pfu DNA polymerase (2.5 U/ µL) | 50 μL | 200 μL |
| Pfu PCR Buffer (10x) | 1.5 mL | 1.5 mL |
| MgCl2 25 mM | 1.5 mL | 1.5 mL |
| High-Q™ dNTPs 10 mM TOTAL | 0.2 mL | 1 mL |
- Broad range of techniques that require high fidelity PCR products such as: cloning, expression of gene of interest, mutagenesis.
- Generation of PCR fragments for blunt cloning.
- Filling 5′ protruding ends.
Parque Científico Tecnológico Rabanales 21, Edificio Aldebarán, Calle Astrónoma Cecilia Payne, 14014 Córdoba. Spain
info@tiarisbiosciences.com
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